Simultaneous Use of MutS and RecA for Suppression of Nonspecific Amplification during PCR

Thermus thermophilus MutS, a thermostable mismatch-recognizing protein, is utilized in PCR to suppress nonspecific amplification by preventing synthesis from mismatched primers. T. thermophilus RecA also decreases nonspecific amplification by promoting proper hybridization between the primer and tem...

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Detalles Bibliográficos
Autores principales: Fukui, Kenji, Kuramitsu, Seiki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3736406/
https://www.ncbi.nlm.nih.gov/pubmed/23970956
http://dx.doi.org/10.1155/2013/823730

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3736406/
https://www.ncbi.nlm.nih.gov/pubmed/23970956
http://dx.doi.org/10.1155/2013/823730

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