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Simultaneous Use of MutS and RecA for Suppression of Nonspecific Amplification during PCR
Thermus thermophilus MutS, a thermostable mismatch-recognizing protein, is utilized in PCR to suppress nonspecific amplification by preventing synthesis from mismatched primers. T. thermophilus RecA also decreases nonspecific amplification by promoting proper hybridization between the primer and tem...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Hindawi Publishing Corporation
2013
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3736406/ https://www.ncbi.nlm.nih.gov/pubmed/23970956 http://dx.doi.org/10.1155/2013/823730 |