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Specific Inhibition of Tumor Cells by Oncogenic EGFR Specific Silencing by RNA interference

Anticancer agents that have minimal effects on normal cells and tissues are ideal cancer drugs. Here, we show specific inhibition of human cancer cells carrying oncogenic mutations in the epidermal growth factor receptor (EGFR) gene by means of oncogenic allele-specific RNA interference (RNAi), both...

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Detalles Bibliográficos
Autores principales: Takahashi, Masaki, Chiyo, Tomoko, Okada, Takashi, Hohjoh, Hirohiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3738543/
https://www.ncbi.nlm.nih.gov/pubmed/23951344
http://dx.doi.org/10.1371/journal.pone.0073214
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author Takahashi, Masaki
Chiyo, Tomoko
Okada, Takashi
Hohjoh, Hirohiko
author_facet Takahashi, Masaki
Chiyo, Tomoko
Okada, Takashi
Hohjoh, Hirohiko
author_sort Takahashi, Masaki
collection PubMed
description Anticancer agents that have minimal effects on normal cells and tissues are ideal cancer drugs. Here, we show specific inhibition of human cancer cells carrying oncogenic mutations in the epidermal growth factor receptor (EGFR) gene by means of oncogenic allele-specific RNA interference (RNAi), both in vivo and in vitro. The allele-specific RNAi (ASP-RNAi) treatment did not affect normal cells or tissues that had no target oncogenic allele, whereas the suppression of a normal EGFR allele by a conventional in vivo RNAi caused adverse effects, i.e., normal EGFR is vital. Taken together, our current findings suggest that specific inhibition of oncogenic EGFR alleles without affecting the normal EGFR allele may provide a safe treatment approach for cancer patients and that ASP-RNAi treatment may be capable of becoming a safe and effective, anticancer treatment method.
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spelling pubmed-37385432013-08-15 Specific Inhibition of Tumor Cells by Oncogenic EGFR Specific Silencing by RNA interference Takahashi, Masaki Chiyo, Tomoko Okada, Takashi Hohjoh, Hirohiko PLoS One Research Article Anticancer agents that have minimal effects on normal cells and tissues are ideal cancer drugs. Here, we show specific inhibition of human cancer cells carrying oncogenic mutations in the epidermal growth factor receptor (EGFR) gene by means of oncogenic allele-specific RNA interference (RNAi), both in vivo and in vitro. The allele-specific RNAi (ASP-RNAi) treatment did not affect normal cells or tissues that had no target oncogenic allele, whereas the suppression of a normal EGFR allele by a conventional in vivo RNAi caused adverse effects, i.e., normal EGFR is vital. Taken together, our current findings suggest that specific inhibition of oncogenic EGFR alleles without affecting the normal EGFR allele may provide a safe treatment approach for cancer patients and that ASP-RNAi treatment may be capable of becoming a safe and effective, anticancer treatment method. Public Library of Science 2013-08-08 /pmc/articles/PMC3738543/ /pubmed/23951344 http://dx.doi.org/10.1371/journal.pone.0073214 Text en © 2013 Takahashi et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Takahashi, Masaki
Chiyo, Tomoko
Okada, Takashi
Hohjoh, Hirohiko
Specific Inhibition of Tumor Cells by Oncogenic EGFR Specific Silencing by RNA interference
title Specific Inhibition of Tumor Cells by Oncogenic EGFR Specific Silencing by RNA interference
title_full Specific Inhibition of Tumor Cells by Oncogenic EGFR Specific Silencing by RNA interference
title_fullStr Specific Inhibition of Tumor Cells by Oncogenic EGFR Specific Silencing by RNA interference
title_full_unstemmed Specific Inhibition of Tumor Cells by Oncogenic EGFR Specific Silencing by RNA interference
title_short Specific Inhibition of Tumor Cells by Oncogenic EGFR Specific Silencing by RNA interference
title_sort specific inhibition of tumor cells by oncogenic egfr specific silencing by rna interference
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3738543/
https://www.ncbi.nlm.nih.gov/pubmed/23951344
http://dx.doi.org/10.1371/journal.pone.0073214
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