An expression system for screening of proteins for glycan and protein interactions

Here we describe a versatile high-throughput expression system that permits genome-wide screening of type 1 membrane and secreted proteins for interactions with glycans and proteins using both cell-expressed and soluble forms of the expressed proteins. Based on Gateway cloning methodology, we have e...

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Detalles Bibliográficos
Autores principales: Otto, Diana M.E., Campanero-Rhodes, Maria A., Karamanska, Rositsa, Powell, Andrew K., Bovin, Nicolai, Turnbull, Jeremy E., Field, Robert A., Blackburn, Jonathan, Feizi, Ten, Crocker, Paul R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3740237/
https://www.ncbi.nlm.nih.gov/pubmed/21211507
http://dx.doi.org/10.1016/j.ab.2010.12.036

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3740237/
https://www.ncbi.nlm.nih.gov/pubmed/21211507
http://dx.doi.org/10.1016/j.ab.2010.12.036

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