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A Novel High Throughput Biochemical Assay to Evaluate the HuR Protein-RNA Complex Formation
The RNA binding protein HuR/ELAVL1 binds to AU-rich elements (AREs) promoting the stabilization and translation of a number of mRNAs into the cytoplasm, dictating their fate. We applied the AlphaScreen technology using purified human HuR protein, expressed in a mammalian cell-based system, to charac...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3741180/ https://www.ncbi.nlm.nih.gov/pubmed/23951323 http://dx.doi.org/10.1371/journal.pone.0072426 |