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A Novel High Throughput Biochemical Assay to Evaluate the HuR Protein-RNA Complex Formation

The RNA binding protein HuR/ELAVL1 binds to AU-rich elements (AREs) promoting the stabilization and translation of a number of mRNAs into the cytoplasm, dictating their fate. We applied the AlphaScreen technology using purified human HuR protein, expressed in a mammalian cell-based system, to charac...

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Detalles Bibliográficos
Autores principales:	D’Agostino, Vito G., Adami, Valentina, Provenzani, Alessandro
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2013
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3741180/ https://www.ncbi.nlm.nih.gov/pubmed/23951323 http://dx.doi.org/10.1371/journal.pone.0072426

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