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Effective Alu Repeat Based RT-Qpcr Normalization in Cancer Cell Perturbation Experiments

BACKGROUND: Measuring messenger RNA (mRNA) levels using the reverse transcription quantitative polymerase chain reaction (RT-qPCR) is common practice in many laboratories. A specific set of mRNAs as internal control reference genes is considered as the preferred strategy to normalize RT-qPCR data. P...

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Detalles Bibliográficos
Autores principales: Rihani, Ali, Van Maerken, Tom, Pattyn, Filip, Van Peer, Gert, Beckers, Anneleen, De Brouwer, Sara, Kumps, Candy, Mets, Evelien, Van der Meulen, Joni, Rondou, Pieter, Leonelli, Carina, Mestdagh, Pieter, Speleman, Frank, Vandesompele, Jo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3743747/
https://www.ncbi.nlm.nih.gov/pubmed/23977142
http://dx.doi.org/10.1371/journal.pone.0071776