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Increased signals from short-wavelength-excited fluorescent molecules using sub-Ti:Sapphire wavelengths
We report the use of an all-solid-state ultrashort pulsed source specifically for two-photon microscopy at wavelengths shorter than those of the conventional Ti:Sapphire laser. Our approach involves sum–frequency mixing of the output from an optical parametric oscillator (λ= 1400–1640 nm) synchronou...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3746106/ https://www.ncbi.nlm.nih.gov/pubmed/23078118 http://dx.doi.org/10.1111/j.1365-2818.2012.03663.x |