Discovery of new Longin and Roadblock domains that form platforms for small GTPases in Ragulator and TRAPP-II

Guanine nucleotide exchange factors (GEFs) control the site and extent of GTPase activity. Longin domains (LDs) are found in many Rab-GEFs, including DENNs, MON1/CCZ1, BLOC-3 and the TRAPP complex. Other GEFs, including Ragulator, contain roadblock domains (RDs), the structure of which is closely re...

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Autores principales: Levine, Timothy P., Daniels, Rachel D., Wong, Louise H., Gatta, Alberto T., Gerondopoulos, Andreas, Barr, Francis A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2013
Materias:
Letter to the Editor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3747258/
https://www.ncbi.nlm.nih.gov/pubmed/23511850
http://dx.doi.org/10.4161/sgtp.24262
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author Levine, Timothy P.
Daniels, Rachel D.
Wong, Louise H.
Gatta, Alberto T.
Gerondopoulos, Andreas
Barr, Francis A.
author_facet Levine, Timothy P.
Daniels, Rachel D.
Wong, Louise H.
Gatta, Alberto T.
Gerondopoulos, Andreas
Barr, Francis A.
author_sort Levine, Timothy P.
collection PubMed
description Guanine nucleotide exchange factors (GEFs) control the site and extent of GTPase activity. Longin domains (LDs) are found in many Rab-GEFs, including DENNs, MON1/CCZ1, BLOC-3 and the TRAPP complex. Other GEFs, including Ragulator, contain roadblock domains (RDs), the structure of which is closely related to LDs. Other GTPase regulators, including mglB, SRX and Rags, use LDs or RDs as platforms for GTPases. Here, we review the conserved relationship between GTPases and LD/RDs, showing how LD/RD dimers act as adaptable platforms for GTPases. To extend our knowledge of GEFs, we used a highly sensitive sequence alignment tool to predict the existence of new LD/RDs. We discovered two yeast Ragulator subunits, and also a new LD in TRAPPC10 that may explain the Rab11-GEF activity ascribed to TRAPP-II.
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spelling pubmed-37472582013-08-27 Discovery of new Longin and Roadblock domains that form platforms for small GTPases in Ragulator and TRAPP-II Levine, Timothy P. Daniels, Rachel D. Wong, Louise H. Gatta, Alberto T. Gerondopoulos, Andreas Barr, Francis A. Small GTPases Letter to the Editor Guanine nucleotide exchange factors (GEFs) control the site and extent of GTPase activity. Longin domains (LDs) are found in many Rab-GEFs, including DENNs, MON1/CCZ1, BLOC-3 and the TRAPP complex. Other GEFs, including Ragulator, contain roadblock domains (RDs), the structure of which is closely related to LDs. Other GTPase regulators, including mglB, SRX and Rags, use LDs or RDs as platforms for GTPases. Here, we review the conserved relationship between GTPases and LD/RDs, showing how LD/RD dimers act as adaptable platforms for GTPases. To extend our knowledge of GEFs, we used a highly sensitive sequence alignment tool to predict the existence of new LD/RDs. We discovered two yeast Ragulator subunits, and also a new LD in TRAPPC10 that may explain the Rab11-GEF activity ascribed to TRAPP-II. Landes Bioscience 2013-04-01 2013-03-19 /pmc/articles/PMC3747258/ /pubmed/23511850 http://dx.doi.org/10.4161/sgtp.24262 Text en Copyright © 2013 Landes Bioscience http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Letter to the Editor
Levine, Timothy P.
Daniels, Rachel D.
Wong, Louise H.
Gatta, Alberto T.
Gerondopoulos, Andreas
Barr, Francis A.
Discovery of new Longin and Roadblock domains that form platforms for small GTPases in Ragulator and TRAPP-II
title Discovery of new Longin and Roadblock domains that form platforms for small GTPases in Ragulator and TRAPP-II
title_full Discovery of new Longin and Roadblock domains that form platforms for small GTPases in Ragulator and TRAPP-II
title_fullStr Discovery of new Longin and Roadblock domains that form platforms for small GTPases in Ragulator and TRAPP-II
title_full_unstemmed Discovery of new Longin and Roadblock domains that form platforms for small GTPases in Ragulator and TRAPP-II
title_short Discovery of new Longin and Roadblock domains that form platforms for small GTPases in Ragulator and TRAPP-II
title_sort discovery of new longin and roadblock domains that form platforms for small gtpases in ragulator and trapp-ii
topic Letter to the Editor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3747258/
https://www.ncbi.nlm.nih.gov/pubmed/23511850
http://dx.doi.org/10.4161/sgtp.24262
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