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The Effect of Primer Choice and Short Read Sequences on the Outcome of 16S rRNA Gene Based Diversity Studies

Different regions of the bacterial 16S rRNA gene evolve at different evolutionary rates. The scientific outcome of short read sequencing studies therefore alters with the gene region sequenced. We wanted to gain insight in the impact of primer choice on the outcome of short read sequencing efforts....

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Detalles Bibliográficos
Autores principales: Ghyselinck, Jonas, Pfeiffer, Stefan, Heylen, Kim, Sessitsch, Angela, De Vos, Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3747265/
https://www.ncbi.nlm.nih.gov/pubmed/23977026
http://dx.doi.org/10.1371/journal.pone.0071360