Size Of Gene Specific Inverted Repeat - Dependent Gene Deletion In Saccharomyces cerevisiae

We describe here an approach for rapidly producing scar-free and precise gene deletions in S. cerevisiae with high efficiency. Preparation of the disruption gene cassette in this approach was simply performed by overlap extension-PCR of an invert repeat of a partial or complete sequence of the targe...

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Detalles Bibliográficos
Autores principales: Lim, Chanyuen, Luhe, Annette Lin, JingYing, Crystal Tear, Balagurunathan, Balaji, Wu, Jinchuan, Zhao, Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3748122/
https://www.ncbi.nlm.nih.gov/pubmed/23977230
http://dx.doi.org/10.1371/journal.pone.0072137

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3748122/
https://www.ncbi.nlm.nih.gov/pubmed/23977230
http://dx.doi.org/10.1371/journal.pone.0072137

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