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Size Of Gene Specific Inverted Repeat - Dependent Gene Deletion In Saccharomyces cerevisiae
We describe here an approach for rapidly producing scar-free and precise gene deletions in S. cerevisiae with high efficiency. Preparation of the disruption gene cassette in this approach was simply performed by overlap extension-PCR of an invert repeat of a partial or complete sequence of the targe...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2013
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3748122/ https://www.ncbi.nlm.nih.gov/pubmed/23977230 http://dx.doi.org/10.1371/journal.pone.0072137 |