CRISPR-assisted editing of bacterial genomes

The targeting of nucleases to specific DNA sequences facilitates genome editing. Recent work demonstrated that the CRISPR-associated (Cas) nuclease Cas9 can be targeted to sequences in vitro simply by modifying a short7 CRISPR RNA (crRNA) guide. Here we use this CRISPR-Cas system to introduce marker...

Descripción completa

Detalles Bibliográficos
Autores principales: Jiang, Wenyan, Bikard, David, Cox, David, Zhang, Feng, Marraffini, Luciano A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3748948/
https://www.ncbi.nlm.nih.gov/pubmed/23360965
http://dx.doi.org/10.1038/nbt.2508

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3748948/
https://www.ncbi.nlm.nih.gov/pubmed/23360965
http://dx.doi.org/10.1038/nbt.2508

Ejemplares similares