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CRISPR-assisted editing of bacterial genomes

The targeting of nucleases to specific DNA sequences facilitates genome editing. Recent work demonstrated that the CRISPR-associated (Cas) nuclease Cas9 can be targeted to sequences in vitro simply by modifying a short7 CRISPR RNA (crRNA) guide. Here we use this CRISPR-Cas system to introduce marker...

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Detalles Bibliográficos
Autores principales:	Jiang, Wenyan, Bikard, David, Cox, David, Zhang, Feng, Marraffini, Luciano A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2013
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3748948/ https://www.ncbi.nlm.nih.gov/pubmed/23360965 http://dx.doi.org/10.1038/nbt.2508

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