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Genome-Wide Exploration of miRNA Function in Mammalian Muscle Cell Differentiation

MiRNAs impact on the control of cell fate by regulating gene expression at the post-transcriptional level. Here, using mammalian muscle differentiation as a model and a phenotypic loss-of-function screen, we explored the function of miRNAs at the genome-wide level. We found that the depletion of a h...

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Autores principales: Polesskaya, Anna, Degerny, Cindy, Pinna, Guillaume, Maury, Yves, Kratassiouk, Gueorgui, Mouly, Vincent, Morozova, Nadya, Kropp, Jeremie, Frandsen, Niels, Harel-Bellan, Annick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3749189/
https://www.ncbi.nlm.nih.gov/pubmed/23991007
http://dx.doi.org/10.1371/journal.pone.0071927
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author Polesskaya, Anna
Degerny, Cindy
Pinna, Guillaume
Maury, Yves
Kratassiouk, Gueorgui
Mouly, Vincent
Morozova, Nadya
Kropp, Jeremie
Frandsen, Niels
Harel-Bellan, Annick
author_facet Polesskaya, Anna
Degerny, Cindy
Pinna, Guillaume
Maury, Yves
Kratassiouk, Gueorgui
Mouly, Vincent
Morozova, Nadya
Kropp, Jeremie
Frandsen, Niels
Harel-Bellan, Annick
author_sort Polesskaya, Anna
collection PubMed
description MiRNAs impact on the control of cell fate by regulating gene expression at the post-transcriptional level. Here, using mammalian muscle differentiation as a model and a phenotypic loss-of-function screen, we explored the function of miRNAs at the genome-wide level. We found that the depletion of a high number of miRNAs (63) impacted on differentiation of human muscle precursors, underscoring the importance of this post-transcriptional mechanism of gene regulation. Interestingly, a comparison with miRNA expression profiles revealed that most of the hit miRNAs did not show any significant variations of expression during differentiation. These constitutively expressed miRNAs might be required for basic and/or essential cell function, or else might be regulated at the post-transcriptional level. MiRNA inhibition yielded a variety of phenotypes, reflecting the widespread miRNA involvement in differentiation. Using a functional screen (the STarS - Suppressor Target Screen – approach, i. e. concomitant knockdown of miRNAs and of candidate target proteins), we discovered miRNA protein targets that are previously uncharacterized controllers of muscle-cell terminal differentiation. Our results provide a strategy for functional annotation of the human miRnome.
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spelling pubmed-37491892013-08-29 Genome-Wide Exploration of miRNA Function in Mammalian Muscle Cell Differentiation Polesskaya, Anna Degerny, Cindy Pinna, Guillaume Maury, Yves Kratassiouk, Gueorgui Mouly, Vincent Morozova, Nadya Kropp, Jeremie Frandsen, Niels Harel-Bellan, Annick PLoS One Research Article MiRNAs impact on the control of cell fate by regulating gene expression at the post-transcriptional level. Here, using mammalian muscle differentiation as a model and a phenotypic loss-of-function screen, we explored the function of miRNAs at the genome-wide level. We found that the depletion of a high number of miRNAs (63) impacted on differentiation of human muscle precursors, underscoring the importance of this post-transcriptional mechanism of gene regulation. Interestingly, a comparison with miRNA expression profiles revealed that most of the hit miRNAs did not show any significant variations of expression during differentiation. These constitutively expressed miRNAs might be required for basic and/or essential cell function, or else might be regulated at the post-transcriptional level. MiRNA inhibition yielded a variety of phenotypes, reflecting the widespread miRNA involvement in differentiation. Using a functional screen (the STarS - Suppressor Target Screen – approach, i. e. concomitant knockdown of miRNAs and of candidate target proteins), we discovered miRNA protein targets that are previously uncharacterized controllers of muscle-cell terminal differentiation. Our results provide a strategy for functional annotation of the human miRnome. Public Library of Science 2013-08-21 /pmc/articles/PMC3749189/ /pubmed/23991007 http://dx.doi.org/10.1371/journal.pone.0071927 Text en © 2013 Harel-Bellan et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Polesskaya, Anna
Degerny, Cindy
Pinna, Guillaume
Maury, Yves
Kratassiouk, Gueorgui
Mouly, Vincent
Morozova, Nadya
Kropp, Jeremie
Frandsen, Niels
Harel-Bellan, Annick
Genome-Wide Exploration of miRNA Function in Mammalian Muscle Cell Differentiation
title Genome-Wide Exploration of miRNA Function in Mammalian Muscle Cell Differentiation
title_full Genome-Wide Exploration of miRNA Function in Mammalian Muscle Cell Differentiation
title_fullStr Genome-Wide Exploration of miRNA Function in Mammalian Muscle Cell Differentiation
title_full_unstemmed Genome-Wide Exploration of miRNA Function in Mammalian Muscle Cell Differentiation
title_short Genome-Wide Exploration of miRNA Function in Mammalian Muscle Cell Differentiation
title_sort genome-wide exploration of mirna function in mammalian muscle cell differentiation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3749189/
https://www.ncbi.nlm.nih.gov/pubmed/23991007
http://dx.doi.org/10.1371/journal.pone.0071927
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