Immunofluorescence labeling of cell surface antigens in Dictyostelium

BACKGROUND: Immunolocalization of cellular antigens typically requires fixation and permeabilization of cells, prior to incubation with antibodies. FINDINGS: Assessing a test protein abundantly present at the cell surface of Dictyostelium cells, we show that in fixed cells, permeabilization extracts...

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Detalles Bibliográficos
Autores principales: Vernay, Alexandre, Cosson, Pierre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3751103/
https://www.ncbi.nlm.nih.gov/pubmed/23937900
http://dx.doi.org/10.1186/1756-0500-6-317

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3751103/
https://www.ncbi.nlm.nih.gov/pubmed/23937900
http://dx.doi.org/10.1186/1756-0500-6-317

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