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Immunofluorescence labeling of cell surface antigens in Dictyostelium
BACKGROUND: Immunolocalization of cellular antigens typically requires fixation and permeabilization of cells, prior to incubation with antibodies. FINDINGS: Assessing a test protein abundantly present at the cell surface of Dictyostelium cells, we show that in fixed cells, permeabilization extracts...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2013
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3751103/ https://www.ncbi.nlm.nih.gov/pubmed/23937900 http://dx.doi.org/10.1186/1756-0500-6-317 |