In silico screening of 393 mutants facilitates enzyme engineering of amidase activity in CalB

Our previously presented method for high throughput computational screening of mutant activity (Hediger et al., 2012) is benchmarked against experimentally measured amidase activity for 22 mutants of Candida antarctica lipase B (CalB). Using an appropriate cutoff criterion for the computed barriers,...

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Detalles Bibliográficos
Autores principales: Hediger, Martin R., De Vico, Luca, Rannes, Julie B., Jäckel, Christian, Besenmatter, Werner, Svendsen, Allan, Jensen, Jan H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2013
Materias:
Biochemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3757469/
https://www.ncbi.nlm.nih.gov/pubmed/24010022
http://dx.doi.org/10.7717/peerj.145

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3757469/
https://www.ncbi.nlm.nih.gov/pubmed/24010022
http://dx.doi.org/10.7717/peerj.145

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