Cargando…

In silico screening of 393 mutants facilitates enzyme engineering of amidase activity in CalB

Our previously presented method for high throughput computational screening of mutant activity (Hediger et al., 2012) is benchmarked against experimentally measured amidase activity for 22 mutants of Candida antarctica lipase B (CalB). Using an appropriate cutoff criterion for the computed barriers,...

Descripción completa

Detalles Bibliográficos
Autores principales:	Hediger, Martin R., De Vico, Luca, Rannes, Julie B., Jäckel, Christian, Besenmatter, Werner, Svendsen, Allan, Jensen, Jan H.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	PeerJ Inc. 2013
Materias:	Biochemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3757469/ https://www.ncbi.nlm.nih.gov/pubmed/24010022 http://dx.doi.org/10.7717/peerj.145

Ejemplares similares

A Computational Methodology to Screen Activities of Enzyme Variants
por: Hediger, Martin R., et al.
Publicado: (2012)

A computational method for the systematic screening of reaction barriers in enzymes: searching for Bacillus circulans xylanase mutants with greater activity towards a synthetic substrate
por: Hediger, Martin R., et al.
Publicado: (2013)

A cytoplasmic peptidoglycan amidase homologue controls mycobacterial cell wall synthesis
por: Boutte, Cara C, et al.
Publicado: (2016)

A Novel CalB-Type Lipase Discovered by Fungal Genomes Mining
por: Vaquero, Maria E., et al.
Publicado: (2015)

Dissecting the evolvability landscape of the CalB active site toward aromatic substrates
por: Santos, Yossef López de los, et al.
Publicado: (2019)

Thermally Stable and Reusable Ceramic Encapsulated and Cross-Linked CalB Enzyme Particles for Rapid Hydrolysis and Esterification
por: Song, Min, et al.
Publicado: (2022)

Thermally Stable and Reusable Silica and Nano-Fructosome Encapsulated CalB Enzyme Particles for Rapid Enzymatic Hydrolysis and Acylation
por: Jang, Woo Young, et al.
Publicado: (2023)

Sortase-mediated immobilization of Candida antarctica lipase B (CalB) on graphene oxide; comparison with chemical approach
por: Moosavi, Faezeh, et al.
Publicado: (2022)

Mutant enzymes challenge all assumptions
por: Nottingham, Ryan M, et al.
Publicado: (2014)

Antibacterial potency of type VI amidase effector toxins is dependent on substrate topology and cellular context
por: Radkov, Atanas, et al.
Publicado: (2022)

AQP9 Expression in Glioblastoma Multiforme Tumors Is Limited to a Small Population of Astrocytic Cells and CD15(+)/CalB(+) Leukocytes
por: Jelen, Sabina, et al.
Publicado: (2013)

CaLB Catalyzed Conversion of ε-Caprolactone in Aqueous Medium. Part 1: Immobilization of CaLB to Microgels
por: Engel, Stefan, et al.
Publicado: (2016)

Geometric Simulation Approach for Grading and Assessing the Thermostability of CALBs
por: Senthilkumar, B., et al.
Publicado: (2016)

Steroidal glycoalkaloids from Solanum nigrum target cytoskeletal proteins: an in silico analysis
por: Ahmad, Rumana
Publicado: (2019)

Discrete analysis of camelid variable domains: sequences, structures, and in-silico structure prediction
por: Melarkode Vattekatte, Akhila, et al.
Publicado: (2020)

In silico structural homology modeling and characterization of multiple N-terminal domains of selected bacterial Tcps
por: Alaidarous, Mohammed
Publicado: (2020)

A Cdk1 phosphomimic mutant of MCAK impairs microtubule end recognition
por: Belsham, Hannah R., et al.
Publicado: (2017)

An Amidase_3 domain-containing N-acetylmuramyl-L-alanine amidase is required for mycobacterial cell division
por: Senzani, Sibusiso, et al.
Publicado: (2017)

Is Promiscuous CALB a Good Scaffold for Designing New Epoxidases?
por: Bordes, Isabel, et al.
Publicado: (2015)

RecA filament sliding on DNA facilitates homology search
por: Ragunathan, Kaushik, et al.
Publicado: (2012)

Correction: RecA filament sliding on DNA facilitates homology search
por: Ragunathan, Kaushik, et al.
Publicado: (2013)

Structure and stability of recombinant bovine odorant-binding protein: I. Design and analysis of monomeric mutants
por: Stepanenko, Olga V., et al.
Publicado: (2016)

Control of the structural landscape and neuronal proteotoxicity of mutant Huntingtin by domains flanking the polyQ tract
por: Shen, Koning, et al.
Publicado: (2016)

In-silico prediction and modeling of the Entamoeba histolytica proteins: Serine-rich Entamoeba histolytica protein and 29 kDa Cysteine-rich protease
por: Manochitra, Kumar, et al.
Publicado: (2017)

Activation-triggered subunit exchange between CaMKII holoenzymes facilitates the spread of kinase activity
por: Stratton, Margaret, et al.
Publicado: (2014)

Paeoniflorin improves functional recovery through repressing neuroinflammation and facilitating neurogenesis in rat stroke model
por: Tang, Hongli, et al.
Publicado: (2021)

In silico structural and functional prediction of African swine fever virus protein-B263R reveals features of a TATA-binding protein
por: Kinyanyi, Dickson, et al.
Publicado: (2018)

Correction: Activation-triggered subunit exchange between CaMKII holoenzymes facilitates the spread of kinase activity
por: Stratton, Margaret, et al.
Publicado: (2014)

RUNX1 facilitates heart failure progression through regulating TGF-β-induced cardiac remodeling
por: Qi, Peng, et al.
Publicado: (2023)

From in silico to in vitro: a trip to reveal flavonoid binding on the Rattus norvegicus Kir6.1 ATP-sensitive inward rectifier potassium channel
por: Trezza, Alfonso, et al.
Publicado: (2018)

A multi-reference poly-conformational method for in silico design, optimization, and repositioning of pharmaceutical compounds illustrated for selected SARS-CoV-2 ligands
por: Alexandrov, Vadim, et al.
Publicado: (2022)

In Silico Prediction of Mutant HIV-1 Proteases Cleaving a Target Sequence
por: Jensen, Jan H., et al.
Publicado: (2014)

Down-regulated NEDD4L facilitates tumor progression through activating Notch signaling in lung adenocarcinoma
por: Lin, Liping, et al.
Publicado: (2022)

An Amidase Contributes to Full Virulence of Sclerotinia sclerotiorum
por: Li, Wei, et al.
Publicado: (2022)

Targeting of the Fun30 nucleosome remodeller by the Dpb11 scaffold facilitates cell cycle-regulated DNA end resection
por: Bantele, Susanne CS, et al.
Publicado: (2017)

HDAC inhibition delays photoreceptor loss in Pde6b mutant mice of retinitis pigmentosa: insights from scRNA-seq and CUT&Tag
por: Dong, Yujie, et al.
Publicado: (2023)

2HybridTools, a handy software to facilitate clone identification and mutation mapping from yeast two-hybrid screening
por: Cauchy, Pierre, et al.
Publicado: (2019)

Data for high-throughput estimation of specific activities of enzyme/mutants in cell lysates through immunoturbidimetric assay of proteins
por: Feng, Yiran, et al.
Publicado: (2017)

393 Vancomycin Dosing in Obese Patients
por: Alquwaizani, Mohammed, et al.
Publicado: (2014)

393. Isavuconazole in the Treatment of Coccidioidal Meningitis
por: Heidari, Arash, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_664qfobttta5ga4ufdv5r7qs3h