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Centrosome repositioning in T cells is biphasic and driven by microtubule end-on capture-shrinkage
T cells rapidly reposition their centrosome to the center of the immunological synapse (IS) to drive polarized secretion in the direction of the bound target cell. Using an optical trap for spatial and temporal control over target presentation, we show that centrosome repositioning in Jurkat T cells...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3760611/ https://www.ncbi.nlm.nih.gov/pubmed/23979719 http://dx.doi.org/10.1083/jcb.201301004 |
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author | Yi, Jason Wu, Xufeng Chung, Andrew H. Chen, James K. Kapoor, Tarun M. Hammer, John A. |
author_facet | Yi, Jason Wu, Xufeng Chung, Andrew H. Chen, James K. Kapoor, Tarun M. Hammer, John A. |
author_sort | Yi, Jason |
collection | PubMed |
description | T cells rapidly reposition their centrosome to the center of the immunological synapse (IS) to drive polarized secretion in the direction of the bound target cell. Using an optical trap for spatial and temporal control over target presentation, we show that centrosome repositioning in Jurkat T cells exhibited kinetically distinct polarization and docking phases and required calcium flux and signaling through both the T cell receptor and integrin to be robust. In “frustrated” conjugates where the centrosome is stuck behind the nucleus, the center of the IS invaginated dramatically to approach the centrosome. Consistently, imaging of microtubules during normal repositioning revealed a microtubule end-on capture-shrinkage mechanism operating at the center of the IS. In agreement with this mechanism, centrosome repositioning was impaired by inhibiting microtubule depolymerization or dynein. We conclude that dynein drives centrosome repositioning in T cells via microtubule end-on capture-shrinkage operating at the center of the IS and not cortical sliding at the IS periphery, as previously thought. |
format | Online Article Text |
id | pubmed-3760611 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-37606112014-03-02 Centrosome repositioning in T cells is biphasic and driven by microtubule end-on capture-shrinkage Yi, Jason Wu, Xufeng Chung, Andrew H. Chen, James K. Kapoor, Tarun M. Hammer, John A. J Cell Biol Research Articles T cells rapidly reposition their centrosome to the center of the immunological synapse (IS) to drive polarized secretion in the direction of the bound target cell. Using an optical trap for spatial and temporal control over target presentation, we show that centrosome repositioning in Jurkat T cells exhibited kinetically distinct polarization and docking phases and required calcium flux and signaling through both the T cell receptor and integrin to be robust. In “frustrated” conjugates where the centrosome is stuck behind the nucleus, the center of the IS invaginated dramatically to approach the centrosome. Consistently, imaging of microtubules during normal repositioning revealed a microtubule end-on capture-shrinkage mechanism operating at the center of the IS. In agreement with this mechanism, centrosome repositioning was impaired by inhibiting microtubule depolymerization or dynein. We conclude that dynein drives centrosome repositioning in T cells via microtubule end-on capture-shrinkage operating at the center of the IS and not cortical sliding at the IS periphery, as previously thought. The Rockefeller University Press 2013-09-02 /pmc/articles/PMC3760611/ /pubmed/23979719 http://dx.doi.org/10.1083/jcb.201301004 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Yi, Jason Wu, Xufeng Chung, Andrew H. Chen, James K. Kapoor, Tarun M. Hammer, John A. Centrosome repositioning in T cells is biphasic and driven by microtubule end-on capture-shrinkage |
title | Centrosome repositioning in T cells is biphasic and driven by microtubule end-on capture-shrinkage |
title_full | Centrosome repositioning in T cells is biphasic and driven by microtubule end-on capture-shrinkage |
title_fullStr | Centrosome repositioning in T cells is biphasic and driven by microtubule end-on capture-shrinkage |
title_full_unstemmed | Centrosome repositioning in T cells is biphasic and driven by microtubule end-on capture-shrinkage |
title_short | Centrosome repositioning in T cells is biphasic and driven by microtubule end-on capture-shrinkage |
title_sort | centrosome repositioning in t cells is biphasic and driven by microtubule end-on capture-shrinkage |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3760611/ https://www.ncbi.nlm.nih.gov/pubmed/23979719 http://dx.doi.org/10.1083/jcb.201301004 |
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