Experimental Pathways towards Developing a Rotavirus Reverse Genetics System: Synthetic Full Length Rotavirus ssRNAs Are Neither Infectious nor Translated in Permissive Cells

At present the ability to create rationally engineered mutant rotaviruses is limited because of the lack of a tractable helper virus-free reverse genetics system. Using the cell culture adapted bovine RV RF strain (G6P6 [1]), we have attempted to recover infectious RV by co-transfecting in vitro tra...

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Detalles Bibliográficos
Autores principales: Richards, James E., Desselberger, Ulrich, Lever, Andrew M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3760874/
https://www.ncbi.nlm.nih.gov/pubmed/24019962
http://dx.doi.org/10.1371/journal.pone.0074328

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3760874/
https://www.ncbi.nlm.nih.gov/pubmed/24019962
http://dx.doi.org/10.1371/journal.pone.0074328

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