Reversible protein affinity-labelling using bromomaleimide-based reagents

Reversible protein biotinylation is readily affected via conjugation with a bromomaleimide-based reagent followed by reductive cleavage. The intermediate biotinylated protein constructs are stable at physiological temperature and pH 8.0. Quantitative reversibility is elegantly delivered under mild c...

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Detalles Bibliográficos
Autores principales: Nathani, Ramiz I., Chudasama, Vijay, Ryan, Chris P., Moody, Paul R., Morgan, Rachel E., Fitzmaurice, Richard J., Smith, Mark E. B., Baker, James R., Caddick, Stephen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2013
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3763775/
https://www.ncbi.nlm.nih.gov/pubmed/23462873
http://dx.doi.org/10.1039/c3ob40239h

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3763775/
https://www.ncbi.nlm.nih.gov/pubmed/23462873
http://dx.doi.org/10.1039/c3ob40239h

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