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Direct Quantification of mRNA and miRNA from Cell Lysates Using Reverse Transcription Real Time PCR: A Multidimensional Analysis of the Performance of Reagents and Workflows

Substantial efforts have been devoted to in vitro testing of candidate chemotherapeutics by profiling transcriptional changes across the collection of NCI-60 cell-lines. A work-flow with reagents that enable the direct quantification of RNA of different molecular sizes simultaneously in the same sam...

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Detalles Bibliográficos
Autores principales: Ho, Yoon Khei, Xu, Wen Ting, Too, Heng Phon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3764000/
https://www.ncbi.nlm.nih.gov/pubmed/24039771
http://dx.doi.org/10.1371/journal.pone.0072463