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The apoptotic effects of sirtuin1 inhibitor on the MCF-7 and MRC-5 cell lines
Sirtuin1 (SIRT1) is an enzyme that deacetylates histones and several nonhistone proteins including p53 during stress and plays an important role in the survival of tumor cells. Hereby, this study describes the potency of salermide as a SIRT1 inhibitor to induce apoptosis in the MCF-7 and MRC-5 cell...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3764679/ https://www.ncbi.nlm.nih.gov/pubmed/24019817 |
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author | Dastjerdi, M Nikbakht Salahshoor, M R Mardani, M Rabbani, M Hashemibeni, B Gharagozloo, M Kazemi, M Esmaeil, N Roshankhah, Sh Golmohammadi, R Mobarakian, M |
author_facet | Dastjerdi, M Nikbakht Salahshoor, M R Mardani, M Rabbani, M Hashemibeni, B Gharagozloo, M Kazemi, M Esmaeil, N Roshankhah, Sh Golmohammadi, R Mobarakian, M |
author_sort | Dastjerdi, M Nikbakht |
collection | PubMed |
description | Sirtuin1 (SIRT1) is an enzyme that deacetylates histones and several nonhistone proteins including p53 during stress and plays an important role in the survival of tumor cells. Hereby, this study describes the potency of salermide as a SIRT1 inhibitor to induce apoptosis in the MCF-7 and MRC-5 cell lines. MCF7 and MRC-5 cell lines were cultured in RPMI-1640 and treated with or without salermide at concentration of 80.56 μmol/L, based on the half-maximal inhibitory concentration (IC50) index at different times (24, 48 and72 h). The IC50 value was established for the salermide in MCF-7. The percentage of apoptotic cells was measured by flow cytometry. Real-time quantitative RT-PCR was performed to estimate the mRNA expression of sirtuin1 in MCF-7 and MRC-5 with salermide at different times. ELISA and Bradford protein techniques were used to detect endogenous levels of total and acetylated p53 protein generated in MCF-7 and MRC-5 cells. Our findings indicated that salermide can induce apoptosis in MCF-7 significantly more effective than MRC-5 cells. We showed that the expression of SIRT1 was dramatically down-regulated by increasing the time of salermide treatment in MCF-7 but not MRC-5 and that the acetylated and total p53 protein levels were increased more in MCF-7 than MRC-5. Salermide, by decreasing the expression of sirtuin1 gene, can induce acetylation of P53 protein and consequently induce significant cell death in MCF-7 that was well tolerated in MRC-5. |
format | Online Article Text |
id | pubmed-3764679 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-37646792013-09-09 The apoptotic effects of sirtuin1 inhibitor on the MCF-7 and MRC-5 cell lines Dastjerdi, M Nikbakht Salahshoor, M R Mardani, M Rabbani, M Hashemibeni, B Gharagozloo, M Kazemi, M Esmaeil, N Roshankhah, Sh Golmohammadi, R Mobarakian, M Res Pharm Sci Original Article Sirtuin1 (SIRT1) is an enzyme that deacetylates histones and several nonhistone proteins including p53 during stress and plays an important role in the survival of tumor cells. Hereby, this study describes the potency of salermide as a SIRT1 inhibitor to induce apoptosis in the MCF-7 and MRC-5 cell lines. MCF7 and MRC-5 cell lines were cultured in RPMI-1640 and treated with or without salermide at concentration of 80.56 μmol/L, based on the half-maximal inhibitory concentration (IC50) index at different times (24, 48 and72 h). The IC50 value was established for the salermide in MCF-7. The percentage of apoptotic cells was measured by flow cytometry. Real-time quantitative RT-PCR was performed to estimate the mRNA expression of sirtuin1 in MCF-7 and MRC-5 with salermide at different times. ELISA and Bradford protein techniques were used to detect endogenous levels of total and acetylated p53 protein generated in MCF-7 and MRC-5 cells. Our findings indicated that salermide can induce apoptosis in MCF-7 significantly more effective than MRC-5 cells. We showed that the expression of SIRT1 was dramatically down-regulated by increasing the time of salermide treatment in MCF-7 but not MRC-5 and that the acetylated and total p53 protein levels were increased more in MCF-7 than MRC-5. Salermide, by decreasing the expression of sirtuin1 gene, can induce acetylation of P53 protein and consequently induce significant cell death in MCF-7 that was well tolerated in MRC-5. Medknow Publications & Media Pvt Ltd 2013 /pmc/articles/PMC3764679/ /pubmed/24019817 Text en Copyright: © Journal of Research in Pharmaceutical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Dastjerdi, M Nikbakht Salahshoor, M R Mardani, M Rabbani, M Hashemibeni, B Gharagozloo, M Kazemi, M Esmaeil, N Roshankhah, Sh Golmohammadi, R Mobarakian, M The apoptotic effects of sirtuin1 inhibitor on the MCF-7 and MRC-5 cell lines |
title | The apoptotic effects of sirtuin1 inhibitor on the MCF-7 and MRC-5 cell lines |
title_full | The apoptotic effects of sirtuin1 inhibitor on the MCF-7 and MRC-5 cell lines |
title_fullStr | The apoptotic effects of sirtuin1 inhibitor on the MCF-7 and MRC-5 cell lines |
title_full_unstemmed | The apoptotic effects of sirtuin1 inhibitor on the MCF-7 and MRC-5 cell lines |
title_short | The apoptotic effects of sirtuin1 inhibitor on the MCF-7 and MRC-5 cell lines |
title_sort | apoptotic effects of sirtuin1 inhibitor on the mcf-7 and mrc-5 cell lines |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3764679/ https://www.ncbi.nlm.nih.gov/pubmed/24019817 |
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