An efficient protocol for the purification and labeling of entire yeast septin rods from E.coli for quantitative in vitro experimentation

BACKGROUND: The detailed understanding of the functions and mechanisms of the actin and microtubuli cytoskeleton depended, besides innovative methods in live cell imaging, on the purification and labeling of its constituents. This allowed researchers to quantitatively measure filament stability, the...

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Detalles Bibliográficos
Autores principales: Renz, Christian, Johnsson, Nils, Gronemeyer, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3765318/
https://www.ncbi.nlm.nih.gov/pubmed/23889817
http://dx.doi.org/10.1186/1472-6750-13-60

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3765318/
https://www.ncbi.nlm.nih.gov/pubmed/23889817
http://dx.doi.org/10.1186/1472-6750-13-60

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