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Evaluation of DNA extraction protocols for Brucella abortus pcr detection in aborted fetuses or calves born from cows experimentally infected with strain 2308

The objective of the present study was to improve the detection of B. abortus by PCR in organs of aborted fetuses from infected cows, an important mechanism to find infected herds on the eradication phase of the program. So, different DNA extraction protocols were compared, focusing the PCR detectio...

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Detalles Bibliográficos
Autores principales: Matrone, M., Keid, L.B., Rocha, V.C.M., Vejarano, M.P., Ikuta, C.Y., Rodriguez, C.A.R., Ferreira, F., Dias, R.A., Ferreira Neto, J.S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768545/
https://www.ncbi.nlm.nih.gov/pubmed/24031391
http://dx.doi.org/10.1590/S1517-838220090003000010