Glass bead transformation method for gram-positive bacteria

A simple, inexpensive and reproducible transformation method was developed for Gram-positive bacteria. It was based on agitation of bacterial protoplasts with glass beads in the presence of DNA and polyethylene glycol. By using this method, introduction of pGK12 into protoplasts of several strains o...

Descripción completa

Detalles Bibliográficos
Autores principales: Rattanachaikunsopon, Pongsak, Phumkhachorn, Parichat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2009
Materias:
General Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768579/
https://www.ncbi.nlm.nih.gov/pubmed/24031442
http://dx.doi.org/10.1590/S1517-838220090004000025

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768579/
https://www.ncbi.nlm.nih.gov/pubmed/24031442
http://dx.doi.org/10.1590/S1517-838220090004000025

Ejemplares similares