Glass bead transformation method for gram-positive bacteria

A simple, inexpensive and reproducible transformation method was developed for Gram-positive bacteria. It was based on agitation of bacterial protoplasts with glass beads in the presence of DNA and polyethylene glycol. By using this method, introduction of pGK12 into protoplasts of several strains o...

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Detalles Bibliográficos
Autores principales: Rattanachaikunsopon, Pongsak, Phumkhachorn, Parichat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2009
Materias:
General Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768579/
https://www.ncbi.nlm.nih.gov/pubmed/24031442
http://dx.doi.org/10.1590/S1517-838220090004000025
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author Rattanachaikunsopon, Pongsak
Phumkhachorn, Parichat
author_facet Rattanachaikunsopon, Pongsak
Phumkhachorn, Parichat
author_sort Rattanachaikunsopon, Pongsak
collection PubMed
description A simple, inexpensive and reproducible transformation method was developed for Gram-positive bacteria. It was based on agitation of bacterial protoplasts with glass beads in the presence of DNA and polyethylene glycol. By using this method, introduction of pGK12 into protoplasts of several strains of Gram-positive bacteria was achieved.
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spelling pubmed-37685792013-09-12 Glass bead transformation method for gram-positive bacteria Rattanachaikunsopon, Pongsak Phumkhachorn, Parichat Braz J Microbiol General Microbiology A simple, inexpensive and reproducible transformation method was developed for Gram-positive bacteria. It was based on agitation of bacterial protoplasts with glass beads in the presence of DNA and polyethylene glycol. By using this method, introduction of pGK12 into protoplasts of several strains of Gram-positive bacteria was achieved. Sociedade Brasileira de Microbiologia 2009 2009-12-01 /pmc/articles/PMC3768579/ /pubmed/24031442 http://dx.doi.org/10.1590/S1517-838220090004000025 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle General Microbiology
Rattanachaikunsopon, Pongsak
Phumkhachorn, Parichat
Glass bead transformation method for gram-positive bacteria
title Glass bead transformation method for gram-positive bacteria
title_full Glass bead transformation method for gram-positive bacteria
title_fullStr Glass bead transformation method for gram-positive bacteria
title_full_unstemmed Glass bead transformation method for gram-positive bacteria
title_short Glass bead transformation method for gram-positive bacteria
title_sort glass bead transformation method for gram-positive bacteria
topic General Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768579/
https://www.ncbi.nlm.nih.gov/pubmed/24031442
http://dx.doi.org/10.1590/S1517-838220090004000025
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