The Carboxyl-Terminal Amino Acids Render Pro-Human LC3B Migration Similar to Lipidated LC3B in SDS-PAGE

LC3 is widely used marker for macroautophagy assays. After translation pro-LC3 is processed by Atg4 to expose C-terminal glycine residue for downstream conjugation reactions to accomplish the conversion of LC3-I to LC3-II. SDS-PAGE based Western blot (Wb) is generally utilized to quantify LC3-II lev...

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Detalles Bibliográficos
Autores principales: Wang, Wei, Chen, Zhixia, Billiar, Timothy R., Stang, Michael T., Gao, Wentao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769297/
https://www.ncbi.nlm.nih.gov/pubmed/24040206
http://dx.doi.org/10.1371/journal.pone.0074222

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769297/
https://www.ncbi.nlm.nih.gov/pubmed/24040206
http://dx.doi.org/10.1371/journal.pone.0074222

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