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Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping

We report a comparative study of two DNA extraction techniques, thermolysis and chemical lysis (CTAB), for molecular identification and genotyping of M. tuberculosis. Forty DNA samples were subjected to PCR and the results demonstrated that with thermolysis it is possible to obtain useful data that...

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Detalles Bibliográficos
Autores principales: Miyata, Marcelo, Santos, Adolfo Carlos Barreto, Mendes, Natália Helena, Cunha, Eunice Atsuko, de Melo, Fernando Augusto Fiúza, Leite, Clarice Queico Fujimura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769851/
https://www.ncbi.nlm.nih.gov/pubmed/24031692
http://dx.doi.org/10.1590/S1517-838220110002000045