Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping

We report a comparative study of two DNA extraction techniques, thermolysis and chemical lysis (CTAB), for molecular identification and genotyping of M. tuberculosis. Forty DNA samples were subjected to PCR and the results demonstrated that with thermolysis it is possible to obtain useful data that...

Descripción completa

Detalles Bibliográficos
Autores principales: Miyata, Marcelo, Santos, Adolfo Carlos Barreto, Mendes, Natália Helena, Cunha, Eunice Atsuko, de Melo, Fernando Augusto Fiúza, Leite, Clarice Queico Fujimura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2011
Materias:
Genetics and Molecular Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769851/
https://www.ncbi.nlm.nih.gov/pubmed/24031692
http://dx.doi.org/10.1590/S1517-838220110002000045
_version_ 1782284044255363072
author Miyata, Marcelo
Santos, Adolfo Carlos Barreto
Mendes, Natália Helena
Cunha, Eunice Atsuko
de Melo, Fernando Augusto Fiúza
Leite, Clarice Queico Fujimura
author_facet Miyata, Marcelo
Santos, Adolfo Carlos Barreto
Mendes, Natália Helena
Cunha, Eunice Atsuko
de Melo, Fernando Augusto Fiúza
Leite, Clarice Queico Fujimura
author_sort Miyata, Marcelo
collection PubMed
description We report a comparative study of two DNA extraction techniques, thermolysis and chemical lysis (CTAB), for molecular identification and genotyping of M. tuberculosis. Forty DNA samples were subjected to PCR and the results demonstrated that with thermolysis it is possible to obtain useful data that enables fast identification and genotyping.
format Online
Article
Text
id pubmed-3769851
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Sociedade Brasileira de Microbiologia
record_format MEDLINE/PubMed
spelling pubmed-37698512013-09-12 Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping Miyata, Marcelo Santos, Adolfo Carlos Barreto Mendes, Natália Helena Cunha, Eunice Atsuko de Melo, Fernando Augusto Fiúza Leite, Clarice Queico Fujimura Braz J Microbiol Genetics and Molecular Microbiology We report a comparative study of two DNA extraction techniques, thermolysis and chemical lysis (CTAB), for molecular identification and genotyping of M. tuberculosis. Forty DNA samples were subjected to PCR and the results demonstrated that with thermolysis it is possible to obtain useful data that enables fast identification and genotyping. Sociedade Brasileira de Microbiologia 2011 2011-06-01 /pmc/articles/PMC3769851/ /pubmed/24031692 http://dx.doi.org/10.1590/S1517-838220110002000045 Text en © Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by-nc/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License
spellingShingle Genetics and Molecular Microbiology
Miyata, Marcelo
Santos, Adolfo Carlos Barreto
Mendes, Natália Helena
Cunha, Eunice Atsuko
de Melo, Fernando Augusto Fiúza
Leite, Clarice Queico Fujimura
Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping
title Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping
title_full Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping
title_fullStr Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping
title_full_unstemmed Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping
title_short Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping
title_sort assessment of the quality of dna extracted by two techniques from mycobacterium tuberculosis for fast molecular identification and genotyping
topic Genetics and Molecular Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769851/
https://www.ncbi.nlm.nih.gov/pubmed/24031692
http://dx.doi.org/10.1590/S1517-838220110002000045
work_keys_str_mv AT miyatamarcelo assessmentofthequalityofdnaextractedbytwotechniquesfrommycobacteriumtuberculosisforfastmolecularidentificationandgenotyping
AT santosadolfocarlosbarreto assessmentofthequalityofdnaextractedbytwotechniquesfrommycobacteriumtuberculosisforfastmolecularidentificationandgenotyping
AT mendesnataliahelena assessmentofthequalityofdnaextractedbytwotechniquesfrommycobacteriumtuberculosisforfastmolecularidentificationandgenotyping
AT cunhaeuniceatsuko assessmentofthequalityofdnaextractedbytwotechniquesfrommycobacteriumtuberculosisforfastmolecularidentificationandgenotyping
AT demelofernandoaugustofiuza assessmentofthequalityofdnaextractedbytwotechniquesfrommycobacteriumtuberculosisforfastmolecularidentificationandgenotyping
AT leiteclaricequeicofujimura assessmentofthequalityofdnaextractedbytwotechniquesfrommycobacteriumtuberculosisforfastmolecularidentificationandgenotyping

Ejemplares similares