High frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

CRISPR RNA-guided endonucleases (RGENs) have rapidly emerged as a facile and efficient platform for genome editing. Here, we use a human cell-based reporter assay to characterize off-target cleavage of Cas9-based RGENs. We find that single and double mismatches are tolerated to varying degrees depen...

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Detalles Bibliográficos
Autores principales: Fu, Yanfang, Foden, Jennifer A., Khayter, Cyd, Maeder, Morgan L., Reyon, Deepak, Joung, J. Keith, Sander, Jeffry D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3773023/
https://www.ncbi.nlm.nih.gov/pubmed/23792628
http://dx.doi.org/10.1038/nbt.2623

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3773023/
https://www.ncbi.nlm.nih.gov/pubmed/23792628
http://dx.doi.org/10.1038/nbt.2623

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