Cargando…

High frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

CRISPR RNA-guided endonucleases (RGENs) have rapidly emerged as a facile and efficient platform for genome editing. Here, we use a human cell-based reporter assay to characterize off-target cleavage of Cas9-based RGENs. We find that single and double mismatches are tolerated to varying degrees depen...

Descripción completa

Detalles Bibliográficos
Autores principales:	Fu, Yanfang, Foden, Jennifer A., Khayter, Cyd, Maeder, Morgan L., Reyon, Deepak, Joung, J. Keith, Sander, Jeffry D.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2013
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3773023/ https://www.ncbi.nlm.nih.gov/pubmed/23792628 http://dx.doi.org/10.1038/nbt.2623

Ejemplares similares

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs
por: Fu, Yanfang, et al.
Publicado: (2014)

Improved Somatic Mutagenesis in Zebrafish Using Transcription Activator-Like Effector Nucleases (TALENs)
por: Moore, Finola E., et al.
Publicado: (2012)

Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing
por: Tsai, Shengdar Q., et al.
Publicado: (2014)

FLASH Assembly of TALENs Enables High-Throughput Genome Editing
por: Reyon, Deepak, et al.
Publicado: (2012)

GUIDE-Seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
por: Tsai, Shengdar Q., et al.
Publicado: (2014)

Heritable and Precise Zebrafish Genome Editing Using a CRISPR-Cas System
por: Hwang, Woong Y., et al.
Publicado: (2013)

Efficient In Vivo Genome Editing Using RNA-Guided Nucleases
por: Hwang, Woong Y., et al.
Publicado: (2013)

Targeted gene disruption in somatic zebrafish cells using engineered TALENs
por: Sander, Jeffry D., et al.
Publicado: (2011)

In silico abstraction of zinc finger nuclease cleavage profiles reveals an expanded landscape of off-target sites
por: Sander, Jeffry D., et al.
Publicado: (2013)

Rapid Mutation of Endogenous Zebrafish Genes Using Zinc Finger Nucleases Made by Oligomerized Pool ENgineering (OPEN)
por: Foley, Jonathan E., et al.
Publicado: (2009)

Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize
por: Lee, Keunsub, et al.
Publicado: (2018)

Evaluation of OPEN Zinc Finger Nucleases for Direct Gene Targeting of the ROSA26 Locus in Mouse Embryos
por: Hermann, Mario, et al.
Publicado: (2012)

Robust, synergistic regulation of human gene expression using TALE activators
por: Maeder, Morgan L., et al.
Publicado: (2013)

Selection-Free Zinc-Finger Nuclease Engineering by Context-Dependent Assembly (CoDA)
por: Sander, Jeffry D., et al.
Publicado: (2010)

Highly efficient generation of heritable zebrafish gene mutations using homo- and heterodimeric TALENs
por: Cade, Lindsay, et al.
Publicado: (2012)

Broad Specificity Profiling of TALENs Results in Engineered Nucleases With Improved DNA Cleavage Specificity
por: Guilinger, John P., et al.
Publicado: (2014)

ZiFiT (Zinc Finger Targeter): an updated zinc finger engineering tool
por: Sander, Jeffry D., et al.
Publicado: (2010)

ZFNGenome: A comprehensive resource for locating zinc finger nuclease target sites in model organisms
por: Reyon, Deepak, et al.
Publicado: (2011)

CIRCLE-seq: a highly sensitive in vitro screen for genome-wide CRISPR-Cas9 nuclease off-targets
por: Tsai, Shengdar Q., et al.
Publicado: (2017)

Targeted Deletion and Inversion of Tandemly Arrayed Genes in Arabidopsis thaliana Using Zinc Finger Nucleases
por: Qi, Yiping, et al.
Publicado: (2013)

Potential high-frequency off-target mutagenesis induced by CRISPR/Cas9 in Arabidopsis and its prevention
por: Zhang, Qiang, et al.
Publicado: (2018)

Engineered CRISPR-Cas9 nucleases with altered PAM specificities
por: Kleinstiver, Benjamin P., et al.
Publicado: (2015)

High frequency modification of plant genes using engineered zinc finger nucleases
por: Townsend, Jeffrey A., et al.
Publicado: (2009)

Measuring and Reducing Off-Target Activities of Programmable Nucleases Including CRISPR-Cas9
por: Koo, Taeyoung, et al.
Publicado: (2015)

Minimizing off-Target Mutagenesis Risks Caused by Programmable Nucleases
por: Ishida, Kentaro, et al.
Publicado: (2015)

Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells
por: Kleinstiver, Benjamin P., et al.
Publicado: (2016)

In vivo mutagenesis of miRNA gene families using a scalable multiplexed CRISPR/Cas9 nuclease system
por: Narayanan, Anand, et al.
Publicado: (2016)

CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans
por: Waaijers, Selma, et al.
Publicado: (2013)

Applications of Alternative Nucleases in the Age of CRISPR/Cas9
por: Guha, Tuhin K., et al.
Publicado: (2017)

CRISPR nuclease off-target activity and mitigation strategies
por: Wienert, Beeke, et al.
Publicado: (2022)

Target-dependent nickase activities of CRISPR-Cas nucleases Cpf1 and Cas9
por: Hua Fu, Becky Xu, et al.
Publicado: (2019)

CRISPR RNA-guided activation of endogenous human genes
por: Maeder, Morgan L, et al.
Publicado: (2013)

An Agrobacterium‐delivered CRISPR/Cas9 system for high‐frequency targeted mutagenesis in maize
por: Char, Si Nian, et al.
Publicado: (2016)

Application of CRISPR/Cas9 Nuclease in Amphioxus Genome Editing
por: Su, Liuru, et al.
Publicado: (2020)

Target DNA mutagenesis-based fluorescence assessment of off-target activity of the CRISPR-Cas9 system
por: Wang, Dan, et al.
Publicado: (2019)

Corrigendum: CRISPR nuclease off-target activity and mitigation strategies
por: Wienert, Beeke, et al.
Publicado: (2023)

Adenoviral vector delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array of human cells
por: Maggio, Ignazio, et al.
Publicado: (2014)

CRISPR GUARD protects off-target sites from Cas9 nuclease activity using short guide RNAs
por: Coelho, Matthew A., et al.
Publicado: (2020)

CRISPR/Cas-Mediated Targeted Mutagenesis in Daphnia magna
por: Nakanishi, Takashi, et al.
Publicado: (2014)

Targeted mutagenesis in rice using CRISPR-Cas system
por: Miao, Jin, et al.
Publicado: (2013)

Cannot write session to /tmp/vufind_sessions/sess_klvjd441k5vba677utd42gk9p3