A Novel Aromatic Carboxylic Acid Inactivates Luciferase by Acylation of an Enzymatically Active Regulatory Lysine Residue

Firefly luciferase (Luc) is widely used as a reporter enzyme in cell-based assays for gene expression. A novel aromatic carboxylic acid, F-53, reported here for the first time, substantially inhibited the enzymatic activity of Luc in a Luc reporter screening. Matrix-assisted laser desorption/ionizat...

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Detalles Bibliográficos
Autores principales: Nakagomi, Madoka, Fujimaki, Nobuko, Ito, Ai, Toda, Takahiro, Fukasawa, Hiroshi, Shudo, Koichi, Tomita, Ryoichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3774628/
https://www.ncbi.nlm.nih.gov/pubmed/24066181
http://dx.doi.org/10.1371/journal.pone.0075445

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3774628/
https://www.ncbi.nlm.nih.gov/pubmed/24066181
http://dx.doi.org/10.1371/journal.pone.0075445

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