Hydrophobic Fluorescent Probes Introduce Artifacts into Single Molecule Tracking Experiments Due to Non-Specific Binding

Single-molecule techniques are powerful tools to investigate the structure and dynamics of macromolecular complexes; however, data quality can suffer because of weak specific signal, background noise and dye bleaching and blinking. It is less well-known, but equally important, that non-specific bind...

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Detalles Bibliográficos
Autores principales: Zanetti-Domingues, Laura C., Tynan, Christopher J., Rolfe, Daniel J., Clarke, David T., Martin-Fernandez, Marisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3774629/
https://www.ncbi.nlm.nih.gov/pubmed/24066121
http://dx.doi.org/10.1371/journal.pone.0074200

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3774629/
https://www.ncbi.nlm.nih.gov/pubmed/24066121
http://dx.doi.org/10.1371/journal.pone.0074200

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