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Characterization of a Self-sufficient Trans-Anethole Oxygenase from Pseudomonas putida JYR-1

A novel flavoprotein monooxygenase, trans-anethole oxygenase (TAO), from Pseudomonas putida JYR-1, which is capable of catalyzing the oxidation of trans-anethole to p-anisaldehyde, was heterologously expressed in E. coli and purified. Enzymatic kinetics of diverse substrates and cofactors revealed t...

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Detalles Bibliográficos
Autores principales: Han, Dongfei, Sadowsky, Michael J., Chong, Youhoon, Hur, Hor-Gil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3774712/
https://www.ncbi.nlm.nih.gov/pubmed/24066043
http://dx.doi.org/10.1371/journal.pone.0073350