pPCV, a versatile vector for cloning PCR products

The efficiency of PCR product cloning depends on the nature of the DNA polymerase employed because amplicons may have blunt-ends or 3′ adenosines overhangs. Therefore, for amplicon cloning, available commercial vectors are either blunt-ended or have a single 3′ overhanging thymidine. The aim of this...

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Detalles Bibliográficos
Autores principales: Janner, Christiane R, Brito, Ana Lívia P, Moraes, Lidia Maria P, Reis, Viviane CB, Torres, Fernando AG
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2013
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3777018/
https://www.ncbi.nlm.nih.gov/pubmed/24058893
http://dx.doi.org/10.1186/2193-1801-2-441

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3777018/
https://www.ncbi.nlm.nih.gov/pubmed/24058893
http://dx.doi.org/10.1186/2193-1801-2-441

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