Segregation of molecules at cell division reveals native protein localization

We introduce a non-intrusive method exploiting post-division single-cell variability to validate protein localization. The results show that Clp proteases, widely reported to form biologically relevant foci, are in fact uniformly distributed inside Escherichia coli cells, and that many commonly used...

Descripción completa

Detalles Bibliográficos
Autores principales: Landgraf, Dirk, Okumus, Burak, Chien, Peter, Baker, Tania A., Paulsson, Johan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3779060/
https://www.ncbi.nlm.nih.gov/pubmed/22484850
http://dx.doi.org/10.1038/nmeth.1955

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3779060/
https://www.ncbi.nlm.nih.gov/pubmed/22484850
http://dx.doi.org/10.1038/nmeth.1955

Ejemplares similares