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Evaluation of Digital PCR for Absolute RNA Quantification

Gene expression measurements detailing mRNA quantities are widely employed in molecular biology and are increasingly important in diagnostic fields. Reverse transcription (RT), necessary for generating complementary DNA, can be both inefficient and imprecise, but remains a quintessential RNA analysi...

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Detalles Bibliográficos
Autores principales: Sanders, Rebecca, Mason, Deborah J., Foy, Carole A., Huggett, Jim F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3779174/
https://www.ncbi.nlm.nih.gov/pubmed/24073259
http://dx.doi.org/10.1371/journal.pone.0075296