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Evaluation of Digital PCR for Absolute RNA Quantification
Gene expression measurements detailing mRNA quantities are widely employed in molecular biology and are increasingly important in diagnostic fields. Reverse transcription (RT), necessary for generating complementary DNA, can be both inefficient and imprecise, but remains a quintessential RNA analysi...
Autores principales: | Sanders, Rebecca, Mason, Deborah J., Foy, Carole A., Huggett, Jim F. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3779174/ https://www.ncbi.nlm.nih.gov/pubmed/24073259 http://dx.doi.org/10.1371/journal.pone.0075296 |
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