High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity

The RNA-programmable Cas9 endonuclease cleaves double-stranded DNA at sites complementary to a 20-base-pair guide RNA. The Cas9 system has been used to modify genomes in multiple cells and organisms, demonstrating its potential as a facile genome-engineering tool. We used in vitro selection and high...

Descripción completa

Detalles Bibliográficos
Autores principales: Pattanayak, Vikram, Lin, Steven, Guilinger, John P., Ma, Enbo, Doudna, Jennifer A., Liu, David R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3782611/
https://www.ncbi.nlm.nih.gov/pubmed/23934178
http://dx.doi.org/10.1038/nbt.2673

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3782611/
https://www.ncbi.nlm.nih.gov/pubmed/23934178
http://dx.doi.org/10.1038/nbt.2673

Ejemplares similares