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High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity
The RNA-programmable Cas9 endonuclease cleaves double-stranded DNA at sites complementary to a 20-base-pair guide RNA. The Cas9 system has been used to modify genomes in multiple cells and organisms, demonstrating its potential as a facile genome-engineering tool. We used in vitro selection and high...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2013
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3782611/ https://www.ncbi.nlm.nih.gov/pubmed/23934178 http://dx.doi.org/10.1038/nbt.2673 |