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Stability-indicating HPLC Method for Simultaneous Determination of Montelukast and Fexofenadine Hydrochloride
A simple, specific, accurate, and stability-indicating reversed-phase high-performance liquid chromatographic method was developed for the simultaneous determination of montelukast and fexofenadine hydrochloride, using a Lichrospher(®) 100, RP-18e column and a mobile phase composed of methanol:0.1%...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2013
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3783746/ https://www.ncbi.nlm.nih.gov/pubmed/24082344 http://dx.doi.org/10.4103/0250-474X.117426 |
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author | Pankhaniya, Mona Patel, Parula Shah, J. S. |
author_facet | Pankhaniya, Mona Patel, Parula Shah, J. S. |
author_sort | Pankhaniya, Mona |
collection | PubMed |
description | A simple, specific, accurate, and stability-indicating reversed-phase high-performance liquid chromatographic method was developed for the simultaneous determination of montelukast and fexofenadine hydrochloride, using a Lichrospher(®) 100, RP-18e column and a mobile phase composed of methanol:0.1% o-phosphoric acid (90:10 v/v), pH 6.8. The retention times of montelukast and fexofenadine hydrochloride were found to be 10.16 and 12.03 min, respectively. Linearity was established for montelukast and fexofenadine hydrochloride in the range of 2-10 μg/ml and 24-120 μg/ml, respectively. The percentage recoveries of montelukast and fexofenadine hydrochloride were found to be in the range of 99.09 and 99.81%, respectively. Both the drugs were subjected to acid and base hydrolysis, oxidation, photolytic, and thermal degradation conditions. The degradation products of montelukast and fexofenadine hydrochloride were well resolved from the pure drug with significant differences in their retention time values. This method can be successfully employed for simultaneous quantitative analysis of montelukast and fexofenadine hydrochloride in bulk drugs and formulations. |
format | Online Article Text |
id | pubmed-3783746 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-37837462013-09-30 Stability-indicating HPLC Method for Simultaneous Determination of Montelukast and Fexofenadine Hydrochloride Pankhaniya, Mona Patel, Parula Shah, J. S. Indian J Pharm Sci Research Paper A simple, specific, accurate, and stability-indicating reversed-phase high-performance liquid chromatographic method was developed for the simultaneous determination of montelukast and fexofenadine hydrochloride, using a Lichrospher(®) 100, RP-18e column and a mobile phase composed of methanol:0.1% o-phosphoric acid (90:10 v/v), pH 6.8. The retention times of montelukast and fexofenadine hydrochloride were found to be 10.16 and 12.03 min, respectively. Linearity was established for montelukast and fexofenadine hydrochloride in the range of 2-10 μg/ml and 24-120 μg/ml, respectively. The percentage recoveries of montelukast and fexofenadine hydrochloride were found to be in the range of 99.09 and 99.81%, respectively. Both the drugs were subjected to acid and base hydrolysis, oxidation, photolytic, and thermal degradation conditions. The degradation products of montelukast and fexofenadine hydrochloride were well resolved from the pure drug with significant differences in their retention time values. This method can be successfully employed for simultaneous quantitative analysis of montelukast and fexofenadine hydrochloride in bulk drugs and formulations. Medknow Publications & Media Pvt Ltd 2013 /pmc/articles/PMC3783746/ /pubmed/24082344 http://dx.doi.org/10.4103/0250-474X.117426 Text en Copyright: © Indian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Pankhaniya, Mona Patel, Parula Shah, J. S. Stability-indicating HPLC Method for Simultaneous Determination of Montelukast and Fexofenadine Hydrochloride |
title | Stability-indicating HPLC Method for Simultaneous Determination of Montelukast and Fexofenadine Hydrochloride |
title_full | Stability-indicating HPLC Method for Simultaneous Determination of Montelukast and Fexofenadine Hydrochloride |
title_fullStr | Stability-indicating HPLC Method for Simultaneous Determination of Montelukast and Fexofenadine Hydrochloride |
title_full_unstemmed | Stability-indicating HPLC Method for Simultaneous Determination of Montelukast and Fexofenadine Hydrochloride |
title_short | Stability-indicating HPLC Method for Simultaneous Determination of Montelukast and Fexofenadine Hydrochloride |
title_sort | stability-indicating hplc method for simultaneous determination of montelukast and fexofenadine hydrochloride |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3783746/ https://www.ncbi.nlm.nih.gov/pubmed/24082344 http://dx.doi.org/10.4103/0250-474X.117426 |
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