The Origin of Biased Sequence Depth in Sequence-Independent Nucleic Acid Amplification and Optimization for Efficient Massive Parallel Sequencing

Sequence Independent Single Primer Amplification is one of the most widely used random amplification approaches in virology for sequencing template preparation. This technique relies on oligonucleotides consisting of a 3′ random part used to prime complementary DNA synthesis and a 5′ defined tag seq...

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Detalles Bibliográficos
Autores principales: Rosseel, Toon, Van Borm, Steven, Vandenbussche, Frank, Hoffmann, Bernd, van den Berg, Thierry, Beer, Martin, Höper, Dirk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3784409/
https://www.ncbi.nlm.nih.gov/pubmed/24086702
http://dx.doi.org/10.1371/journal.pone.0076144

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3784409/
https://www.ncbi.nlm.nih.gov/pubmed/24086702
http://dx.doi.org/10.1371/journal.pone.0076144

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