In vitro affinity screening of protein and peptide binders by megavalent bead surface display

The advent of protein display systems has provided access to tailor-made protein binders by directed evolution. We introduce a new in vitro display system, bead surface display (BeSD), in which a gene is mounted on a bead via strong non-covalent (streptavidin/biotin) interactions and the correspondi...

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Detalles Bibliográficos
Autores principales: Diamante, Letizia, Gatti-Lafranconi, Pietro, Schaerli, Yolanda, Hollfelder, Florian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Original Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3785251/
https://www.ncbi.nlm.nih.gov/pubmed/23980186
http://dx.doi.org/10.1093/protein/gzt039

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3785251/
https://www.ncbi.nlm.nih.gov/pubmed/23980186
http://dx.doi.org/10.1093/protein/gzt039

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