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In vitro affinity screening of protein and peptide binders by megavalent bead surface display
The advent of protein display systems has provided access to tailor-made protein binders by directed evolution. We introduce a new in vitro display system, bead surface display (BeSD), in which a gene is mounted on a bead via strong non-covalent (streptavidin/biotin) interactions and the correspondi...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2013
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3785251/ https://www.ncbi.nlm.nih.gov/pubmed/23980186 http://dx.doi.org/10.1093/protein/gzt039 |