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A Novel Helper Phage Enabling Construction of Genome-Scale ORF-Enriched Phage Display Libraries
Phagemid-based expression of cloned genes fused to the gIIIP coding sequence and rescue using helper phages, such as VCSM13, has been used extensively for constructing large antibody phage display libraries. However, for randomly primed cDNA and gene fragment libraries, this system encounters readin...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3785514/ https://www.ncbi.nlm.nih.gov/pubmed/24086469 http://dx.doi.org/10.1371/journal.pone.0075212 |