A Novel Helper Phage Enabling Construction of Genome-Scale ORF-Enriched Phage Display Libraries

Phagemid-based expression of cloned genes fused to the gIIIP coding sequence and rescue using helper phages, such as VCSM13, has been used extensively for constructing large antibody phage display libraries. However, for randomly primed cDNA and gene fragment libraries, this system encounters readin...

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Detalles Bibliográficos
Autores principales: Gupta, Amita, Shrivastava, Nimisha, Grover, Payal, Singh, Ajay, Mathur, Kapil, Verma, Vaishali, Kaur, Charanpreet, Chaudhary, Vijay K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3785514/
https://www.ncbi.nlm.nih.gov/pubmed/24086469
http://dx.doi.org/10.1371/journal.pone.0075212

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