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Sensitive, Simultaneous Quantitation of Two Unlabeled DNA Targets Using a Magnetic Nanoparticle–Enzyme Sandwich Assay

[Image: see text] We report herein the development of a simple, sensitive colorimetric magnetic nanoparticle (MNP)–enzyme-based DNA sandwich assay that is suitable for simultaneous, label-free quantitation of two DNA targets down to 50 fM level. It can also effectively discriminate single-nucleotide...

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Detalles Bibliográficos
Autores principales: Zhang, Yue, Pilapong, Chalermchai, Guo, Yuan, Ling, Zhenlian, Cespedes, Oscar, Quirke, Philip, Zhou, Dejian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2013
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3789254/
https://www.ncbi.nlm.nih.gov/pubmed/23971744
http://dx.doi.org/10.1021/ac402081u