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Generalized bacterial genome editing using mobile group II introns and Cre-lox

Efficient bacterial genetic engineering approaches with broad-host applicability are rare. We combine two systems, mobile group II introns (‘targetrons’) and Cre/lox, which function efficiently in many different organisms, into a versatile platform we call GETR (Genome Editing via Targetrons and Rec...

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Detalles Bibliográficos
Autores principales: Enyeart, Peter J, Chirieleison, Steven M, Dao, Mai N, Perutka, Jiri, Quandt, Erik M, Yao, Jun, Whitt, Jacob T, Keatinge-Clay, Adrian T, Lambowitz, Alan M, Ellington, Andrew D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: European Molecular Biology Organization 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3792343/
https://www.ncbi.nlm.nih.gov/pubmed/24002656
http://dx.doi.org/10.1038/msb.2013.41